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Safety Data Sheet (SDS) Biotin Anti-mouse CD25 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD25 Antibody
1110015 50 µg $60.00       
1110020 500 µg $205.00       
Clone: PC61
Isotype: Rat IgG1, λ
Reactivity: Mouse
Immunogen: IL-2-dependent cytolytic mouse T-cell clone B6.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, in vitro blocking of IL-2 binding to low- and high-affinity receptors1-4, growth inhibition of IL-2-dependent T-cell lines1-4, in vivo depletion of CD25+CD4+ Treg cells5-8,10, and immunohistochemical staining of acetone-fixed frozen sections2. PC61 antibody recognizes a different epitope than 3C7 antibody .

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
9. Fukishima A, et al. 2006. Biochem Biophys Res Commun. 339:1048. PubMed
10. León-Ponte M, et al. 2007. Blood 109:3139.
11. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
12. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.

Con A-stimulated C57BL/6 splenocytes (3

Con A-stimulated C57BL/6 splenocytes (3 days) stained with PC 61 biotin, followed by Sav-PE



Description:

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Other Names: IL-2Rα, Ly-43, p55, Tac
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor
Ligand Receptor: IL-2
Antigen References:

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.