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Safety Data Sheet (SDS) PerCP Anti-mouse CD902 Antibody     Product Data Sheet (PDF)    
PerCP Anti-mouse CD90.2 Antibody
1126605 25 µg $95.00       
1126610 100 µg $240.00       
Clone: 30-H12
Isotype: Rat IgG2b, κ
Reactivity: Mouse, Does not react with Thy-1.1 (CD90.1)
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The CD90.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 is recommended prior to immunofluorescent staining.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Application References:

1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
6. Hardy B, et al. 2005. Int. Immunol. 17:615.
7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed

C57BL/6 splenocytes stained with purified

C57BL/6 splenocytes stained with purified 30-H12 PerCP



Description:

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Other Names: Thy-1.2
Structure: Ig superfamily, 25-35 kD
Distribution: Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice
Function: Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand Receptor: CD45
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.