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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD309 VEGFR2 Flk-1 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD309 (VEGFR2, Flk-1) Antibody
1209585 25 µg $125.00       
1209590 100 µg $300.00       
Clone: 89B3A5
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Rat-1 cells transfected with full-length mouse Flk
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Kaburn N, et al. 1997. Development 124:2039.
2. Farren MR, et al. 2014. Sci Signal. 18:313. PubMed
3. Harbuzariu A, et al. 2014. J Vasc Surg. 59:1686. PubMed

FLK-1 transfected cells stained with

FLK-1 transfected cells stained with 89B3A5 PerCP/Cy5.5



Description:

The 89B3A5 antibody recognizes mouse CD309 also known as vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, and fetal liver kinase-1. Flk-1 is a member of the tyrosine protein kinase family, sub-family CSF-1/PDGF, that contains a single pass transmembrane receptor with a protein kinase domain and seven immunoglobulin-like domains in the extracellular region. Flk-1 is expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels. Flk-1 is a receptor for VEGF or VEGFC; ligand binding plays a key role in vascular development and vascular permeability. The 89B3A5 antibody has been shown to be useful for flow cytometry.

Other Names: Vascular endothelial growth factor receptor 2, VEGFR2, KDR, protein tyrosine kinase receptor flk-1, fetal liver kinase-1
Structure: Member of the tyrosine protein kinase family, sub-family CSF-1/PDGF. Single pass transmembrane receptor containing a protein kinase domain and seven immunoglobulin-like domains.
Distribution: Expressed at high levels in adult heart, lung, kidney, brain, and skeletal muscle; other tissues express at lower levels.
Function: Receptor for VEGF or VEGFC. Ligand binding plays a key role in vascular development and vascular permeability.
Ligand Receptor: VEGF, VEGFC
Antigen References:

1. Patterson C, et al. 1995. J. Biol. Chem. 270:23111.
2. Quinn TP, et al. 1993. Proc. Natl. Acad. Sci. USA 90:7533.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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