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Safety Data Sheet (SDS) Purified Anti-mouse CD1d CD11 Ly-38 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD1d (CD1.1, Ly-38) Antibody
1217510 500 µg $215.00       
Clone: 1B1
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC, IP - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3.

This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
2. Brozovic S, et al. 2004. Nat. Med. 10:535.
3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with purified 1B1, followed anti-rat IgG FITC



Description:

CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.


This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Other Names: CD1, CD1.1, Ly-38
Structure: 48 kD glycoprotein, Ig superfamily
Distribution: leukocytes, dendritic cells, epithelial cells, thymocytes
Function: Antigen presentation
Ligand Receptor: similar to MHC class I, associate with ß-microglobulin
Antigen References:

1. Brudin N, et al. 1998. J. Immunol. 161:3271.
2. Amano M, et al. 1998. J. Immunol. 161:1710.
3. Brossay L, et al. 1997. J. Immunol. 159:1216.
4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113.
5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.