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Safety Data Sheet (SDS) Purified Anti-mouse CD14 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse CD14 Antibody
1216510 500 µg $215.00       
Clone: Sa14-2
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

BALB/c peritoneal macrophages stained with

BALB/c peritoneal macrophages stained with Sa14-2 purified, followed by anti-rat IgG FITC



Description:

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.

Other Names: LPS receptor
Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: macrophages, dendritic cells, kupffer cells, hepatocytes
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand Receptor: LPS
Antigen References:

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.