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Safety Data Sheet (SDS) APC Anti-mouse CD21/CD35 CR2/CR1 Antibody     Product Data Sheet (PDF)    
APC Anti-mouse CD21/CD35 (CR2/CR1) Antibody
1217055 25 µg $95.00       
1217060 100 µg $270.00       
Clone: 7E9
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: CD35/CFA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Boackle S, et al. 2001 Immunity 15:775.
2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with 7E9 APC



Description:

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Other Names: CR2/CR1
Structure: 145 kD/190 kD type I transmembrane glycoprotein
Distribution: B cells, follicular dendritic cells
Function: Signal transduction
Ligand Receptor: Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Antigen References:

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.