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Safety Data Sheet (SDS) PE Anti-mouse CD86 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD86 Antibody
1125525 50 µg $95.00       
1125530 200 µg $245.00       
Clone: PO3
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: BALB/c mouse B leukemia cell line BCL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
2. Nuriya S, et al. 1996. Int. Immunol. 8:917.
3. Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
4. Saito K, et al. 1998. J. Immunol. 160:4225.
5. Nakajima A, et al. 1998. J. Immunol. 161:1901.
6. Ma XT, et al. 2006. Cancer Research 66:1169.
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
9. Giroux M, et al. 2007. J. Immunol. 179:4492.
10. Planes R, et al. 2014. J Virol. 88:55672. PubMed

LPS-stimulated (3 days) BALB/c splenocytes

LPS-stimulated (3 days) BALB/c splenocytes stained with PO3 PE



Description:

CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.

Other Names: B7-2, B70, Ly-58
Structure: Ig superfamily, 80 kD
Distribution: B and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor: CD28, CD152 (CTLA-4)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.