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Safety Data Sheet (SDS) Biotin Anti-mouse CD49d Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD49d Antibody
1118015 50 µg $60.00       
1118020 500 µg $220.00       
Clone: R1-2
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: AKR/Cum mouse spontaneous T lymphoma line TK1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The R1-2 antibody has been shown to partially block CD49d mediated interactions and, in combination with the 9C10 (MFR4.B) antibody, completely blocks VCAM-1 binding to VLA-4. Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and T cell costimulation1-4, immunoprecipitation1, and immunohistochemistry of frozen sections5.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated R1-2, followed by Sav-PE



Description:

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.

Other Names: α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Structure: Integrin family, associates with integrin β1 or β7, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand Receptor: VCAM-1, MAdCAM-1, fibronectin
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994 J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.