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Safety Data Sheet (SDS) Biotin Anti-mouse/rat/human CD27 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse/rat/human CD27 Antibody
1221025 50 µg $75.00       
1221030 500 µg $245.00       
Clone: LG.3A10
Isotype: Armenian Hamster IgG
Reactivity: Mouse, Rat, Human
Immunogen: Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: functional assay1,3 and immunohistomecial staining of acetone-fixed frozen sections2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Gravestein LA, et al. 1995. Int. Immunol. 7:551. (FA)
2. Gravestein LA, et al. 1996. J. Exp. Med. 184:675. (IHC)
3. Takeda K, et al. 2000. J. Immunol. 164:1741. (FA)
4. Welner RS, et al. 2009. J. Immunol. 183:7768. PubMed
5. Vicetti Miguel RC, et al. 2012. J. Immunol. 189:3449. PubMed
6. White CA, et al. 2014. J Immunol. 193:5933. PubMed

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with biotinylated LG.3A10, followed by Sav-PE



Description:

CD27 is also known as S152 and T14. A member of the tumor necrosis factor receptor (TNFR) superfamily, it is a 45 kD protein expressed on peripheral T cells, memory B cells, NK cells, and thymocyte subset. Through its ligand, CD70, CD27 plays a key role in T cell and B cell interactions. Additionally, ligation of CD27 on naïve T cells may be important in their maturation to effector cells.

Other Names: T14, S152, Tp55, TNFRSF7
Structure: TNFR superfamily, 45 kD
Distribution: Peripheral blood T cells, memory B cells, NK cells, thymocyte subset
Function: Costimulatory signal for T and B cell activation, T cell development
Ligand Receptor: CD70
Interaction: TRAF2, TRAF5, CD27-binding protein (Siva)
Antigen References:

1. Hintzen RQ, et al. 1994. Immunol. Today 15:307.
2. Akiba H, et al. 1998. J. Biol. Chem. 273:13353.
3. Kobata T, et al. 1995. P. Natl. Acad. Sci. USA 92:11249.
4. Hendriks J, et al. 2000. Nat. Immunol. 1:433
5. Hintzen RQ, et al. 1994. Int. Immunol. 6:477
6. Camerini D, et al. 1991. J. Immunol. 147:3165