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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD69 Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD69 Antibody
1122605 25 µg $95.00       
1122610 100 µg $270.00       
Clone: H1.2F3
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse dendritic epidermal T cell line Y245
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (λ) light chain.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application References:

1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
2. Sobel ES, et al. 1993. J. Immunol. 150:673.
3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
7. Lee JW, et al. 2006. Nature Immunol. 8:181.
8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
9. Jordan JM, et al. 2008. 76:3717. PubMed
10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed

PMA + ionomycin-stimulated (6 hrs)

PMA + ionomycin-stimulated (6 hrs) C57BL/6 splenocytes stained with H1.2F3 PerCP/Cy5.5



Description:

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Other Names: Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Structure: C-type lectin, 27/33 kD
Distribution: Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets
Function: Lymphocyte activation
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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