The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634. 2. Nakagawa Y, et al. 2012. Nat Immunol. 25:11. PubMed
BALB/c mouse splenocytes stained with YTS156.7.7 FITC
CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
Ig superfamily, CD8β chain, 34 kD
Majority of thymocytes, cytotoxic T cell subsets
Co-receptor of TCR
MHC class I
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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