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Safety Data Sheet (SDS) PerCP/Cy55 Anti-mouse CD127 IL-7Ralpha Antibody     Product Data Sheet (PDF)    
PerCP/Cy5.5 Anti-mouse CD127 (IL-7Rα) Antibody
1205565 25 µg $95.00       
1205570 100 µg $270.00       
Clone: SB/199
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: mouse pre-B cell line 1A9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 , is recommended prior to immunofluorescent staining.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application References: 1. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
2. Kouro T, et al. 2002. Blood 100:3672.
3. Maeda K, et al. 2005. Blood 106:879.
4. Diao J, et al. 2004. J. Immunol. 173:1826.
C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with CD3 (145-2C11) APC and SB/199 PerCP/Cy5.5



Description: CD127 is a 60-90 kD type I transmembrane glycoprotein, also known as IL-7 receptor α chain or IL-7Rα. It forms heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
Other Names: IL-7 receptor α chain, IL-7Rα
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand Receptor: IL-7
Antigen References: 1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:9125.
2. He YW and Malek TR. 1998. Crit Rev. Immunol. 18:503.
3. Huster K M,et al. 2004. Proc. Natl. Acad. Sci. USA. 101:5610.
4. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.

This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
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