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Safety Data Sheet (SDS) Alexa Fluor 700 Anti-mouse TCR Beta Chain Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 700 Anti-mouse TCR β Chain Antibody
1146115 25 µg $90.00       
1146120 100 µg $190.00       
Clone: H57-597
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Affinity purified TCR from mouse DO-11.10 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

H57-597 is a hamster mAb directed to an epitope of the C region of TCR β chain12. The H57-597 antibody does not cross-react with γ/δ TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate α/β TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Application References:

1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® 700



Description:

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Other Names: TCR-β chain, TCR-β, β-TCR
Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound-MHC class I and II
Antigen References:

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.