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Safety Data Sheet (SDS) Purified Anti-human/mouse CXCR7 Antibody     Product Data Sheet (PDF)    
Purified Anti-human/mouse CXCR7 Antibody
2255510 100 µg $220.00       
Clone: 8F11-M16
Isotype: Mouse IgG2b, κ
Reactivity: Human, mouse
Immunogen: Human CXCR7 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
ICFC - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: Intracellular staining for flow cytometry.2

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Soehnlein O, et al. 2013. EMBO Mol. Med. 5:471. (FC) PubMed
2. Saad ST, et al. 2014. PLoS One 9(1). (ICFC) PubMed
3. Balabanian K, et al. 2012. J. Transl. Med. 10:251. (FC) PubMed

CXCR7 transfected L1.2 cells were

CXCR7 transfected L1.2 cells were stained with purified CXCR7 (clone 8F11-M16) (filled histogram) or purified mouse IgG2b APC isotype control (open histogram), followed by anti-mouse IgG PE.



Description:

CXCR7, also known as RDC1, belongs to a subgroup of C-X-C chemokine receptors, which are part of a large protein family of G protein-coupled receptors (GPCR).  CXCR7 binds with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC, which regulates the trafficking and activation of leukocytes. It is also a co-receptor for the entry of HIV-1. Binding of ligand to CXCR7 induces proliferation and migration of immature neurons, glia and their precursors.  CXCR7 expression occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells.  Surface expression of CXCR7 was also reported to be on tumor cells, activated endothelial cells, fetal liver cells, and other cell types.

Other Names: Chemokine (C-X-C motif) receptor 7, RDC1, CMKOR1, GPR159
Structure: G Protein-Coupled Receptor super family
Distribution: Monocytes, B cells, T cells and mature dendritic cells
Ligand Receptor: CXCL12/SDF-1 and CXCL11/I-TAC
Regulation: Regulates the trafficking and activation of leukocytes
Cellular Sources: On a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells
Antigen References:

1. Burns JM, et al. 2006. J. Exp. Med. 203:2201.
2. Infantino S, et al. 2006. J. Immunol. 176:2197.