The antibody was purified by affinity chromatography.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested WB - Reported in the literature
Additional reported application (for the relevant formats) include: Western blotting and functional assay.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Matsumoto K, et al. 2004. J. Immunol. 172:2530. 2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed 3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Con A (3-day) activated C57BL/6 mouse splenocytes stained with RMP1-30 PE
CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.
Programmed Death-1, CD279
Ig superfamily, 50-55 kD
Subset of double negative thymocytes, activated T and B cells
Lymphocyte clonal selection, peripheral tolerance
PD-L1 (B7-H1), PD-L2
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Agata Y, et al. 1996. Int. Immunol. 8:765. 3. Nishimura H, et al. 2001. Science 291:319. 4. Ishida Y, et al. 1992. EMBO J. 11:3887.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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