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Safety Data Sheet (SDS) PE Anti-mouse CD105 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD105 Antibody
1202035 25 µg $80.00       
1202040 100 µg $175.00       
Clone: MJ7/18
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: inflamed mouse skin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications include: immunoprecipitation, Western blotting, and immunofluorescence histochemistry or immunohistochemistry of acetone-fixed frozen sections2-4.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Ge AZ and Butcher EC. 1994. Gene 138:201.
2. Baluk P, et al. 2003. Am. J. Pathol. 163:1801. (IHC)
3. Takahashi T, et al. 2003. Mol. Cell Biol. 23:1817. (IHC)
4. Savinov AY, et al. 2003. J. Exp. Med. 197:643. (IHC)

BEND.3, mouse endothelial cells stained

BEND.3, mouse endothelial cells stained with MJ7/18 PE



Description:

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and upregulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF-β1, TGF-β3 and modulates TGF-β signaling by interacting with TGF-β receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2, and BMP-7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging and prognosis, and has therapeutic potential for some solid tumors and other angiogenic diseases.

Other Names: Endoglin
Structure: Type I integral membrane protein, homodimer, TGF-β type III receptor family member
Distribution: Endothelial cells, activated monocytes, macrophages, stromal cells, some cytotrophoblast
Function: Suppresses TGF-β signaling, angiogenesis
Ligand Receptor: TGF-β1, TGF-β3
Antigen References:

1. Gougos A and M. Letarte 1988. J. Immunol. 141:1925.
2. Cheifetz S, et al. 1992. J. Bio. Chem. 267:19027.
3. Barbara NP, et al. 1999. J. Bio. Chem. 274:584.
4. Lastres P, et al. 1992. Eur. J. Immunol. 22:393.
5. Duff S, et al. 2003. FASEB J. 17:984.
6. Warrington K, et al. 2005. Anticancer Res. 25:185.