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Safety Data Sheet (SDS) PE Anti-mouse CD252 OX40L Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD252 (OX40L) Antibody
1144025 50 µg $90.00       
1144030 200 µg $255.00       
Clone: RM134L
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Rat NRK-52E cell line transfected with mouse OX-40L.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The RM134L antibody can block the costimulatory activity of OX40L. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3, and in vivo and in vitro blocking of OX-40L-OX-40 functional interaction1,2,4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Akiba H, et al. 1999. J. Immunol. 162:7058. (Block)
2. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
3. Hoshino A, et al. 2003. Eur. J. Immunol. 33:861. (IHC)
4. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Block)
5. Zheng X, et al. 2010. Invest Ophthalmol Vis Sci. 51:3076. PubMed

C57BL/6 splenocytes were stimulated with

C57BL/6 splenocytes were stimulated with anti-mouse IgM and CD40 antibodies for 3 days, then stained with OX40L (clone RM134L) PE (filled histogram) or rat IgG2b PE isotype control (open histogram).



Description:

CD252 is a 35 kD member of the TNF superfamily and is also known as OX40 ligand, OX40L, and CD134L. The CD252 is expressed on activated B cells and antigen presenting cells. CD252 interacts with OX40 antigen (CD134), expressed predominantly on activated T cells to increase proliferation and IL-2 production and to enhance proliferation and immunoglobulin secretion in activated B cells.

Other Names: OX-40L, CD134L, CD252, TNFSF4
Structure: NGF/TNF superfamily, 35 kD
Distribution: Activated B cells, antigen presenting cells
Function: B-cell/T-cell interaction, costimulation
Ligand Receptor: OX40(CD134)
Antigen References:

1. Akiba H, et al. 1999. J. Immunol. 162:7058.
2. Stüber E, et al. 1995. Immunity 2:507.
3. Baum PR, et al. 1994. EMBO J. 13:3992.