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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse TCR Vbeta81 82 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse TCR Vβ8.1, 8.2 Antibody
1192070 100 µg $200.00       
Clone: KJ16-133.18
Isotype: Rat IgG2a, κ
Reactivity: mouse
Immunogen: TCR receptor from T cell hybridoma DO-11.10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

BALB/c splenocytes stained with KJ16-133,18

BALB/c splenocytes stained with KJ16-133,18 Alexa Fluor® 647



Description:

The TCR Vβ 8 family consists of three members, namely Vβ8.1, 8.2 and 8.3. KJ16-133.18 reacts with Vβ8.1, 8.2 only, but not Vβ8.3. Most mouse strains have about 20% Vβ 8+ T cells in the periphery.However, C57BR, C57L, SJL, SWR (Tcra haplotype) and RIII strains (Tcrc haplotype) do not express Vβ8 due to a gene deletion at the Vβ 8 family loci.Other strains expressing endogenous super-antigen, such as Mls-1a have significant reduced number of Vβ 8.1 cells in the periphery.Exogenous superantigen, such as staphylococcal enterotoxin B, stimulates lymphocytes bearing Vβ 8 and selectively eliminate those T cells in vivo.

Other Names: T cell receptor Vβ8.1, 8.2 chain, TCR Vβ8.1, 8.2
Structure: TCR is a 85-kd hetero-dimer composed of alpha and beta chain. The mouse Vβ8 family has three members, namely Vβ8.1, 8.2 and 8.3.
Distribution: Expressed on α:β T cells in mouse thymus, spleen and other lymphoid organs.
Function: Recognition of peptide/ MHC
Interaction: CD3, CD4, CD8
Antigen References:

1. Haskins K, et al. 1984. J. Exp. Med. 160:452
2. Behlke MA, et al. 1986. P. Natl. Acad. Sci. USA 83:767
3. Fairchild S, et al. 1992. 36:189
4. White J, et al. 1989. Cell 56:27


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