The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-paraffin)
BALB/c mouse splenocytes stained with TY/11.8 PE and CD3 (145-2C11) APC
CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
69 kD GPI-anchored surface protein
Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Nucleoside 5' monophosphate
Expression is regulated by the development and maturation of leukocytes
1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981. 2. Resta R, et al. 1998. Immunol. Rev. 161:95.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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