The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen sections, and in vivo blocking of CD30 binding to CD30 ligand (CD153)1-3,5.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519. 2. Seko Y, et al. 2001. J. Pathol. 195:593. 3. Akiba H, et al. 2000. J. Exp. Med. 191:375. 4. Zhang H and GG Meadows 2005. J. Leukocyte Biol. 78:1070.PubMed 5. Zeiser R, et al. 2007. Blood 109:2225.
TPA-stimulated (20 hrs) splenocytes stained with purified RM153, then detected with biotinylated anti-rat IgG followed by Sav-PE
CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.
CD30 ligand, CD30L, TNFSF8
TNF superfamily, 39-40 kD
Activated T cells
T-B cell costimulation
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Horie R, et al. 1998. Semin. Immunol. 106:457. 3. Shimozato O, et al. 1999. Biochem. Biophys. Res. Commun. 256:519. 4. Shanebeck KD, et al. 1995. Eur. J. Immunol. 25:2147. 5. Bowen MA, et al. 1996. J. Immunol. 156:442.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Sony, the Sony logo, Sony Biotechnology Inc. and logo, iCyt, the iCyt logo EC800 and SY3200 are trademarks of Sony Corporation. Alexa Fluor® and Pacific Blue™ are registered trademarks of and licensed under patents assigned to Life Technologies Corporation. Cy and CyDye are trademarks of GE Healthcare Limited. iCyt products containing Cy5, Cy5.5 or Cy7 are manufactured under license from GE Healthcare under U.S. Patent Numbers 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. Brilliant Violet™, Brilliant Violet 421™, BV421™, Brilliant Violet 570™, BV570™, Brilliant Violet 605™, BV605™, Brilliant Violet 650™, and BV650™, Brilliant Violet 510™, BV510™, Brilliant Violet 711™, BV711™, Brilliant Violet 785™ and BV785™ are trademarks of Sirigen Group Ltd. PE-Dazzle 594™ is a trademark of Biolegend. All other trademarks are property of their respective owners. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.