Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Peterson DJ, et al. 1987. Mol. Immunol. 24:1281. (IHC, IP, Block) 2. Tellides G, et al. 1987. Transplant Proc. 19:4231. (Block) 3. Digness AU, et al. 1996. Exp.Cell Res. 225:422. (Block)
Con A-stimulated LOU rat splnocytes (3days) stained with biotinylated OX-39, followed by Sav-PE
CD25 is a 55 kD glycoprotein also known as IL-2 receptor α chain. It is broadly expressed on activated T and B cells, a subset of thymic and splenic dendritic cells, and intestinal epithelial cells in the rat. IL-2 is a critical cytokine involved in lymphocyte proliferation and clonal expansion. IL-2 signaling requires the high affinity IL-2 receptor composed of IL-2 receptor chains α, β, and γ. The OX-39 antibody weakly inhibits IL-2 binding to the IL-2 receptor and has been shown to block some IL-2 mediated responses in vitro.
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