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Safety Data Sheet (SDS) FITC Anti-mouse TCR DO1110 Antibody     Product Data Sheet (PDF)    
FITC Anti-mouse TCR DO11.10 Antibody
1192525 25 µg $55.00       
1192530 100 µg $100.00       
Clone: KJ1-26
Isotype: Mouse IgG2a, κ
Reactivity: Mouse
Immunogen: DO11.10 T hybridoma cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed

DO11.10 T-hybridoma cells stained with

DO11.10 T-hybridoma cells stained with KJ1-26 FITC



Description:

DO11.10 is specific for chicken ovalbumin (aa 323-339) in combination with I-Ad MHC class II complex. KJ1-26 was reported to block the antigen response of DO11.10 T cell hybridomas in vitro.

Other Names: DO11.10 Clonotypic T Cell Receptor
Structure: 80-90 kD TCR expressed on DO11.10 T hybridomas
Distribution: Clonotypic TCR
Ligand Receptor: Ovalbumin peptide (aa 323-339) in the context of mouse MHC class II I-Ad
Antigen References:

1. Haskins K, et al. 1983. J. Exp. Med. 157:1149.
2. Marrack P, et al. 1983. J. Exp. Med. 158:1635.