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Safety Data Sheet (SDS) PE Anti-mouse CD2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD2 Antibody
1100535 50 µg $95.00       
1100540 200 µg $245.00       
Clone: RM2-5
Isotype: Rat IgG2b, λ
Reactivity: Mouse
Immunogen: BALB/c mouse thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)

C57BL/6 mouse splenocytes stained with

C57BL/6 mouse splenocytes stained with RM2-5 PE



Description:

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Other Names: LFA-2, T11, Ly-37, SRBC-R
Structure: Ig superfamily, 45-58 kD
Distribution: T cells, thymocytes, NK cells, B cells
Function: T cell activation, adhesion
Ligand Receptor: CD48, lck
Antigen References:

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.