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Safety Data Sheet (SDS) FITC Anti-rat Myeloid Lineage Antibody     Product Data Sheet (PDF)    
FITC Anti-rat Myeloid Lineage Antibody
1625515 100 µg $125.00       
Clone: OX-82
Isotype: Mouse IgG1, κ
Reactivity: Rat myeloid cells
Immunogen: Anemic rat bone marrow
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

Crook K, et al. 1996. Dev Immunol 4:235

LOU rat bone marrow cells

LOU rat bone marrow cells stained with OX-82 FITC (gated on myeloid cell population)



Description:

The OX-82 reacts with a 35 kD molecules expressed on myeloid cells and stromal elements from a variety of tissues in the adult rat. This antibody is useful in isolating pluripotent stem cells from rat bone marrow and fetal liver when used in combination with other antibodies.

Other Names: OX-82
Structure: A 35 kD molecule expressed on myeloid cells and stroma cells from different tissues of adult rat.
Distribution: On myeloid cells and stromal elements from a variety of tissues in the adult rat. Rat hematopoietic stem cells are CD71-CD90+ and OX-82+.
Antigen References:

1. Crook K, et al. 1996. Dev Immunol 4:235