The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
The B20.1 antibody recognizes most members of the Vα2 TCR subfamily in mice having the a, b, and c haplotypes.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. 2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. 3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed 4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated B20.1, followed by Sav-PE
The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ). TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.
T cell receptor Vα 2
On a subset of T cells
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
TCR recognizes MHC/peptide complex
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
1. Kubo RT, et al. 1989. J. Immunol. 142:2736 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399
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