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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ2 Antibody
1239535 25 µg $85.00       
1239540 100 µg $170.00       
Clone: B20.6
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Soluble TCR from mouse CTL clone CW3/1.1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Necker A, et al. 1991. Eur J. Immunol. 21:3035
2. Hodes RJ, et al. 1993. J Immunol 150:1422

C57BL/6 mouse splenocytes were stained

C57BL/6 mouse splenocytes were stained with CD3 APC and TCR Vβ2 (clone B20.6) PE (top) or rat IgG2a, κ PE isotype control (bottom).





Description:

The B20.6 antibody recognizes V beta 2 TCR of all strains tested. V beta 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.

Other Names: T cell receptor Vβ 2 [vb2]
Structure: TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution: On a subset of T cells
Function: Recognize MHC/peptide complex on the antigen-presenting cells.
Ligand Receptor: TCR recognizes MHC/peptide complex
Interaction: Associate with V alpha and CD3 complex to form TCR complexes expressing on cell surface.
Antigen References:

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Necker A, et al. 1991. Eur. J. Immunol. 21:3035