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Safety Data Sheet (SDS) PE Anti-mouse FR4 Folate Receptor 4 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse FR4 (Folate Receptor 4) Antibody
1225535 25 µg $95.00       
Clone: TH6
Isotype: Rat IgG2b, κ
Reactivity: Mouse Folate Receptor 4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1.Yamaguchi T et al. 1007:Immunity 27(1)145

BALB/c mouse splenocytes stained with

BALB/c mouse splenocytes stained with TH6 PE and CD3 (145-2C11 APC)



Description:

Folate receptor 4 (FR4) is a surface receptor for folic acid (Vitamin B9).It was recently reported that FR4 is highly and constitutively expressed on mouse CD4+ CD25+ natural regulatory T cells (Treg).The high expression of this surface marker on Treg can be used in combination with CD4 and CD25 to distinguish Treg from other types of T cells.Since FR4 is a surface marker,this antibody may be used in combination with anti-CD4 and/or CD25 to sort living T reg cells for functional studies.

Other Names: Folate Receptor 4 , FBP, FR delta
Structure: 28 kd protein consisting of 244 amino acids. Heavily glycosylated
Distribution: Expressed on mouse thymocytes and spleen cells. Highly expressed on natural regulatory (Treg) cells
Function: Mediate calcium-independent homophlic cell-cell adhesion
Ligand Receptor: Folic acid and its derivative
Antigen References:

1. Yamaguchi T et al 2007. Immunity 27:145
2. Spiegelstein O et al. 2000 Gene 258:117