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Safety Data Sheet (SDS) PE Anti-rat CD134 OX-40 Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD134 (OX-40) Antibody
1622540 100 µg $190.00       
Clone: OX-40
Isotype: Mouse IgG2b, κ
Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

Al-Shamkhani A, et al. 1996. Eur J. Immunol. 26:1695

Con A-stimulated LOU rat splenocytes

Con A-stimulated LOU rat splenocytes (Day 3) stained with anti-rat CD3 (1F4) Alexa Fluor® 647 and OX-40 PE



Description:

CD134 (OX-40 receptor)is a 50 kd type I membrane protein, expressed on activated T cells.It binds to the OX-40 ligand (CD252) to provide a co-stimulatory signal that is independent of CD28.It is reported to block binding of CD134 and CD252.

Other Names: OX-40 receptor , TNFRSF4, ACT35, OX40
Structure: 50 kd type I integral membrane protein, member of NGFR/TNFR superfamily.
Distribution: Expressed mainly on activated CD4+ and CD8+ T cells.
Function: Binds to the OX-40 ligand (CD252) to provide a co-stimulatory signal for T cells.
Ligand Receptor: CD252 (OX-40 ligand)
Antigen References:

1. Al-Shamkhani A et al. 1996. Eur. J. Immunol. 26:1695
2. Paterson DJ et al. 1987. Mol Immunol 24:1281