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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse TCR Valpha2 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse TCR Vα2 Antibody
1239060 100 µg $190.00       
Clone: B20.1
Isotype: Rat IgG2a, λ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The B20.1 antibody recognizes most members of the Vα2 TCR subfamily in mice having the a, b, and c haplotypes.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed

BALB/c splenocytes stained with B20.1

BALB/c splenocytes stained with B20.1 Alexa Fluor® 647



Description:

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Other Names: T cell receptor Vα 2
Distribution: On a subset of T cells
Function: In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Ligand Receptor: TCR recognizes MHC/peptide complex
Interaction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Antigen References:

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399


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