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Safety Data Sheet (SDS) PE Anti-mouse/human Galectin-3 Mac-2 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse/human Galectin-3 (Mac-2) Antibody
1233530 200 µg $270.00       
Clone: Gal397
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse
Immunogen: Recombinant (partial), amino acids 151-251
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Application Notes:

Galectin-3 will work for either FC or ICFC applications depending on whether it is on the surface or located intracellularly.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Human peripheral blood monocytes intracellular

Human peripheral blood monocytes intracellular stained with Gal397 PE



Description:

Galectin-3 (galactose-specific lectin 3) is a 30-32 kD glycoprotein, also know as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.

Other Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Structure: 30-32 kD glycoprotein, animal β-galactoside-binding protein family
Distribution: Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells
Function: Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand Receptor: Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Antigen References:

1. Kim HRC, et al. 1999. Cancer Res. 59:4148
2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
3. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395
4. Joo GH, et al. 2001. J. Leukoc. Biol. 69:555