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Safety Data Sheet (SDS) Purified Anti-mouse/human Galectin-3 Mac-2 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse/human Galectin-3 (Mac-2) Antibody
1233505 50 µg $65.00       
1233510 500 µg $215.00       
Clone: Gal397
Isotype: Mouse IgG1, κ
Reactivity: Human, Mouse
Immunogen: Recombinant (partial), amino acids 151-251
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

WB - Quality tested
IF, FC, ICFC - Validated

Application Notes:

Galectin-3 will work for either FC or ICFC applications depending on whether it is on the surface or located intracellularly.

Recommended Usage:

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested working dilution is 0.5-1.0 µg/ml antibody dilution buffer for each mini-gel.pdf" target="_blank">immunofluorescent staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Total cell lysate from MCF7

Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.

HeLa cells were stained with

HeLa cells were stained with purified anti-Galectin 3 (Gal397) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).



Description:

Galectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.

Other Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Structure: 250 amino acids with a predicted molecular weight of 26 kD.
Distribution: Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells
Function: Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand Receptor: Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Antigen References:

1. Kim HRC, et al. 1999. Cancer Res. 59:4148
2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
3. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395
4. Joo GH, et al. 2001. J. Leukoc. Biol. 69:555