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Safety Data Sheet (SDS) Purified Anti-mouse/human Mac-2 Galectin-3 Antibody     Product Data Sheet (PDF)    
Purified Anti-mouse/human Mac-2 (Galectin-3) Antibody
1227005 50 µg $65.00       
1227010 500 µg $215.00       
Clone: M3/38
Isotype: Rat IgG2a, κ
Reactivity: Mouse, Human
Immunogen: Raised against galectin-3 of mouse origin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested
IF, WB, IP - Validated
IHC, ELISA - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-3.7

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
8. Usategui A, et al. 2013. Ann Rheum Dis. 72:2018. PubMed (IF)
9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
10. Reales E, et al. 2015. J Cell Sci. 128:2261. PubMed

BALB/c peritoneal macrophages stained with

BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC

HeLa cells were stained with

HeLa cells were stained with purified anti-Galectin 3 (M3/38) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).



Description:

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Other Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Antigen References:

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.