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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse/human Mac-2 Galectin-3 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse/human Mac-2 (Galectin-3) Antibody
1227040 100 µg $220.00       
Clone: M3/38
Isotype: Rat IgG2a, κ
Reactivity: Mouse, Human
Immunogen: Raised against galectin-3 of mouse origin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
IHC-P - Validated
IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections3-6, Western blotting2, immunoprecipitation1,2, immunofluorescence7,8, and ELISA9.

Clone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-3.7

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5-10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221. (FC, IP)
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
4. Jacob N, et al. 2011. J. Immunol. 186:4984. (IHC) PubMed
5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
8. Usategui A, et al. 2013. Ann Rheum Dis. PubMed (IF)
9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
10. Kouo T, et al. 2015. Cancer Immunol Res. 3:412. PubMed

Thioglycolate-elicited BALB/c mouse peritoneal macrophages

Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with M3/38 Alexa Fluor® 647



Description:

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other beta-galactosides but differ in their affinity for more complex saccharides.

Other Names: Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Antigen References:

1. Ho MK. and Springer TA. 1982. J. Immunol. 128:1221.
2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731.


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