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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse Notch 4 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse Notch 4 Antibody
1242060 100 µg $290.00       
Clone: HMN4-14
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Notch4-Fc recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Application References:

1. Moriyama Y,et al.2008. Intl J. Immunol. 20:763.

FN4/CHO (mouse Notch-4) transfected cell

FN4/CHO (mouse Notch-4) transfected cell line stained with HMN4-14 Alexa Fluor® 647



Description:

The Notch receptors and their ligands are highly conserved from invertebrates to mammals.The Delta-like 1 (Dll1) is one of the four or five Notch ligands identified.The binding to Notch receptor results in the proteolysis of Notch and movement of intracellular portion of Notch into the nucleus.This translocation triggers a series of signaling process.Delta-like 1 is reported to be essential for the maintenance of marginal zone B cells in normal mice and engagement of Notch 1 by Dll 1 promotes differentiation of B lymphocytes to antibody-secreting cells.

Other Names: Proto-oncogene of int-3
Structure: Type 1 transmembrane receptor with a large extracellular domain consisting of 29 -36 EGF( epidermal growth factor) and three LNR (Lin12-Notch) repeats.
Distribution: Notch 4 is preferentially expressed in endothelial cells.
Function: Regulation of cell development, including myogenesis, neurogenesis, and lymphocyte development. Notch 4 seems to play a crucial role in vasculogenesis and angiogenesis.
Ligand Receptor: Jagged 1, Jagged2, Delta-like 1, Delta-like3 and Delta-like 4
Antigen References:

1.Ehebauer MT,et al. 2006. Biochem J.392:13.
2.Shimizu K,et al. 2000.Mol Cell Biology. 20:18
3. Tanigaki K,et al.2007. Nature immunol. 8:451.


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