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Safety Data Sheet (SDS) PE/Cy7 Anti-rat CD4 Antibody     Product Data Sheet (PDF)    
PE/Cy7 Anti-rat CD4 Antibody
1607575 25 µg $120.00       
1607580 100 µg $270.00       
Clone: W3/25
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistryof acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with W3/25 PE/CY7



Description:

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Other Names: T4, L3T4, W3/25
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen References:

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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