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Safety Data Sheet (SDS) PE Anti-mouse CD40 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD40 Antibody
1223045 25 µg $80.00       
1223050 100 µg $220.00       
Clone: 3/23
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: Recombinant mouse CD40 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
2. Bourgeois C, et al. 2002. Science 297:2060.

BALB/c splenocytes stained with 3/23

BALB/c splenocytes stained with 3/23 PE



Description:

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Other Names: Bp50, TNFRSF5
Structure: 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution: Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
Function: Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand Receptor: CD154 on T cells
Antigen References:

1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.