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Safety Data Sheet (SDS) Biotin Anti-mouse DC Marker 33D1 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse DC Marker (33D1) Antibody
1224515 50 µg $65.00       
1224520 500 µg $245.00       
Clone: 33D1
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Mouse dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunofluorescence3 and immunohistochemical staining of acetone-fixed frozen sections4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Crowley MT, et al. 1990. J. Immunol. Methods 133:55.
2. Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161.
3. Steinman RM, et al. 1983. J. Exp. Med. 157:613. (IF)
4. Fischer HG, et al. 2000. J. Immunol. 164:4826. (IHC)

BALB/c splenocytes stained with I-A/I-E

BALB/c splenocytes stained with I-A/I-E (M5/114) FITC and biotinylated 33D1, followed by Sav-PE



Description:

33D1 antigen, also known as DCIR2 (dendritic cell inhibitory receptor 2), is reported to be a dendritic cell marker on subpopulation DC in the spleen, thymus, and Peyer's patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.

Other Names: Dendritic Cell Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)
Structure: The antigen recognized by 33D1 is yet unknown.
Distribution: 33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.
Function: GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.