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Safety Data Sheet (SDS) PE Anti-mouse 4-1BB Ligand CD137L Antibody     Product Data Sheet (PDF)    
PE Anti-mouse 4-1BB Ligand (CD137L) Antibody
1135525 50 µg $95.00       
Clone: TKS-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: BALB/c mouse B lymphoma line 2PK-3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The TKS-1 antibody inhibits binding of soluble 4-1BB to 4-1BBL in vitro. Additional reported applications (for the relevant formats) include: in vitro blocking the binding of 4-1BB to 4-1BBL1. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-rat IgG second step , followed by SAv-PE ).

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Akiba H, et al. 2000. J. Exp. Med. 191:375. (Block)
2. Futagawa T, et al. 2002. Int. Immunol. 14:275.
3. Nishimoto H, et al. 2005. Blood 106:4241. (FC) PubMed

Mouse B cell line 2PK3

Mouse B cell line 2PK3 stained with TKS-1 PE



Description:

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily present on antigen-presenting cells (dendritic cells and macrophages) and activated B and T lymphocytes. 4-1BBL interacts with 4-1BB (CDw137) to induce T and B cell costimulation.

Other Names: 4-1BBL, CD137L, TNFSF9
Structure: NGF/TNF superfamily, 97 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand Receptor: 4-1BB (CDw137)
Antigen References:

1. Pollok KE, et al. 1995. Eur. J. Immunol. 25:488.
2. DeBenedette MA, et al. 1997. J. Immunol. 158:551.
3. Pollok KE, et al. 1993. J. Immunol. 150:771.
4. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
5. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.