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Safety Data Sheet (SDS) Biotin Anti-mouse CD34 Antibody     Product Data Sheet (PDF)    
Biotin Anti-mouse CD34 Antibody
1243015 50 µg $80.00       
1243020 500 µg $270.00       
Clone: HM34
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse CD34 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
NIH/3T3 cell line stained with

NIH/3T3 cell line stained with HM34 biotin, followed by Sav-PE



Description:

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.

Other Names: Mucosialin
Structure: Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells
Function: Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand Receptor: L-selectin, and others.
Antigen References:

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175