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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD34 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD34 Antibody
1243025 25 µg $100.00       
1243030 100 µg $220.00       
Clone: HM34
Isotype: Armenian Hamster IgG
Reactivity: Mouse
Immunogen: Mouse CD34 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
NIH/3T3 cell line stained with

NIH/3T3 cell line stained with HM34 Alexa Fluor® 647



Description:

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.

Other Names: Mucosialin
Structure: Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells
Function: Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand Receptor: L-selectin, and others.
Antigen References:

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175


This product is provided under an agreement between Life Technologies Corporation and Sony Biotechnology Inc. and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications, and corresponding non-U.S. equivalents, owned by and/or licensed to Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research and/or development conducted by the buyer (whether the buyer is an academic or for profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to (1) use of the product or its components in manufacturing, (2) use of the product or its components for therapeutic, diagnostic or prophylactic purposes, or (3) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Molecular Probes Detection Technologies, Attn: Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.