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Safety Data Sheet (SDS) FITC Anti-rat CD4 Domain 1 Antibody     Product Data Sheet (PDF)    
FITC Anti-rat CD4 (domain 1) Antibody
1617025 100 µg $125.00       
1617030 500 µg $270.00       
Clone: OX-38
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: Rat thymocyte glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The OX-38 antibody has been shown to bind the same CD4 epitope as the W3/25 antibody, and a different epitope than the OX-35 antibody. Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections and blocking function1.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:

1. Pettigrew GJ, et al. 1998. J. Immunol. 161:1292.

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-38 FITC



Description:

CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.

Other Names: L3T4, T4
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen References:

1. Pettigrew GJ, et al. 1998. J. Immunol. 161:1292.