Sony Biotechnology Inc. Sony Biotechnology Inc.cart
About Us Products What's New Library News Calendar Contact Us Place Order
Safety Data Sheet (SDS) PE Anti-rat RT1A Antibody     Product Data Sheet (PDF)    
PE Anti-rat RT1A Antibody
1626040 100 µg $190.00       
Clone: OX-18
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: RT1B-depleted membrane glycoproteins isolated from Wistar rat spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (For the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, immnoprecipitation, and blocking function

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Hellstrom M, et al. 2014. Acta Biomater. 10:5034. PubMed

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-18 PE



Description:

The rat monomorphic MHC class I antigen RT1A is expressed on nucleated cells (except neurons and glial cells) and erythrocytes. RT1A is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Other Names: MHC Class I, RT1.A
Distribution: Nucleated cells and erythrocytes