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Safety Data Sheet (SDS) PE Anti-rat CD45RC Antibody     Product Data Sheet (PDF)    
PE Anti-rat CD45RC Antibody
1620035 50 µg $110.00       
Clone: OX-22
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: PHA-activated rat lymph node cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (origin U.S.)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2, 3 and formalin-fixed paraffin-embedded tissue sections.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Woollett GR, et al. Eur. J. Immunol. 15:168.
2. Spickett GP, et al. 1983. J. Exp. Med. 158:795.
3. Mason DW, et al. 1983. Immunol. Rev. 74:57.

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-22 PE



Description:

CD45RC is a 200-220 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant (exon C) of the tyrosine phosphatase CD45. The CD45RC isoform is expressed on B cells, majority of CD8+ T cells and a subset of CD4+ T cells, but not on myeloid cells. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens, including CD1, CD2, CD3, and CD4. CD45 is involved in signaling lymphocytes activation.

Other Names: CD45