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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-rat CD4 Domain 2 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-rat CD4 (domain 2) Antibody
1616560 100 µg $220.00       
Clone: OX-35
Isotype: Mouse IgG2a, κ
Reactivity: Rat
Immunogen: MLR generated rat T helper lymphocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The OX-35 antibody has been shown to bind a different CD4 epitope than the W3/25 and OX-38 antibodies. Additional reported applications (for the relevant formats) include: immunohistochemical staining2.

Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Application References:

1. May E, et al. 2003. J. Immunol. 170:1099.
2. Hansson AS, et al. 1999. J. Clin. Invest. 104:589. (IHC)

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-35 Alexa Fluor® 647



Description:

CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.

Other Names: L3T4, T4
Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, T cell subset
Function: TCR co-receptor, T cell activation
Ligand Receptor: MHC class II molecule
Antigen References:

1. May E, et al. 2003. J. Immunol. 170:1099.
2. Hansson AS, et al. 1999. J Clin Invest. 104:589


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