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Safety Data Sheet (SDS) PE Anti-mouse CD137 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse CD137 Antibody
1130525 50 µg $90.00       
1130530 200 µg $255.00       
Clone: 17B5
Isotype: Syrian Hamster IgG
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Additional reported applications (for the relevant formats) include: in vitro blocking of 4-1BB-mediated T cell proliferation2.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Lee SW, et al. 2005. J. Immunol. 174:6803. (FC)
2. Zheng G, et al. 2004. J. Immunol. 173:2428. (Block)
3. Zheng H and G. G. Meadows 2005. J. Leukocyte Biol. 78:1070. (FC) PubMed
4. Nishimoto H, et al. 2005. Blood 106:4241. (FC)
5. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
6. Nemeth T, et al. 2014. Fungal Genet Biol. 65:48. PubMed

Con A-stimulated (3 days) splenocytes

Con A-stimulated (3 days) splenocytes stained with 17B5 PE



Description:

CDw137 is a 39 kD TNF-receptor superfamily member also known as 4-1BB, TNFRSF9, Ly-63, and ILA (Induced by Lymphocyte Activation). CDw137 is expressed as monomers, dimers, and tetramers on activated T and B cells. Interaction of CDw137 with its ligand, 4-1BBL (CDw137L), is important in T-B cell costimulation. The 17B5 antibody has been reported to block 4-1BBL costimulated T cell proliferation.

Other Names: 4-1BB, TNFRSF9, Ly-63, ILA (Induced by Lymphocyte Activation), CDw137
Structure: TNF-R superfamily, 39 kD
Distribution: Activated T cells and B cells
Function: T-B cell costimulation
Ligand Receptor: 4-1BB ligand (CDw137L)
Antigen References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Alderson MR, et al. 1994. Eur. J. Immunol. 24:2219.
3. Schwarz H, et al. 1996. Blood 87:2839.