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Safety Data Sheet (SDS) Purified Anti-rat CD147 Antibody     Product Data Sheet (PDF)    
Purified Anti-rat CD147 Antibody
1623005 100 µg $95.00       
Clone: OX-47
Isotype: Mouse IgG1, κ
Reactivity: Rat
Immunogen: T blasts from a mixed lymphocyte reaction
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC, WB - Reported in the literature

Application Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and Western blotting2


Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Glenn M, et al. 1995. Circulation 92:1919. (IHC)
2. Betsuyaku T, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:600. (WB)

LOU rat splenocytes stained with

LOU rat splenocytes stained with purified OX-47, followed by anti-mIgG1 PE


CD147 is a 54 kD type I integral transmembrane protein, also called extracellular metalloproteinase inducer (EMMMPRIN) or M6 in humans, Basigin or gp42 in mouse, and OX-47 or CE-9 in rat. It is a heavily glycosylated Ig superfamily member and is expressed widely in hematopoietic and non-hematopoietic tissues. CD147 is an adhesion molecule and, through interaction with integrins, plays an important role in fetal, neuronal, blood cells and extracellular matrix development.

Other Names: OX-47, CE-9
Structure: Single chain type I transmembrane 58 kd heavily glycosylated protein. N terminal extracellular part consists of two C2 type Ig-like domains which are highly glycosylated. Recently reported: isoform with an additional membrane-distal Ig-like domain.
Distribution: CD147 is expressed at varying levels in many cells types, including haematopoietic, epithelial, and endothelial cells. Expressed on immature thymocytes and at varying levels on spleen cells , bone marrow cells and macrophages.
Function: CD147 is implicated in multiple functions, such as matrix metalloproteinase induction, cell adhesion, retinal cell development, HIV attachment, embryonic development and T cell activation.
Ligand Receptor: cyclophilin
Antigen References:

1. Glenn M, et al. 1995. Circulation 92:1919.
2. Betsuyaku T, et al. 2003. Am. J. Respir. Cell Mol. Biol. 28:600.