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Safety Data Sheet (SDS) Alexa Fluor 647 Anti-mouse CD226 DNAM-1 Antibody     Product Data Sheet (PDF)    
Alexa Fluor® 647 Anti-mouse CD226 (DNAM-1) Antibody
1244040 100 µg $290.00       
Clone: 10E5
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Immunogen: Th1 polarized T cell clones
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Application References:

1. Dardalhon V, et al. 2005. J. Immunol. 175:1558
2. Huang Z, et al. 2011. J Immunol. 186:3421. PubMed

C57BL/6 splenocytes stained with CD8

C57BL/6 splenocytes stained with CD8 (53-6.7) FITC and 10E5 Alexa Fluor® 647



Description:

CD226 (DNAM-1) is constitutively expressed on native CD8+ cells and on CD4+ T cells, macrophages and NK cells.This antibody (10E5) was reported to bind about 40% of inactivated CD4+ cells and binds only to differentiated Th1 cells, but not to Th2 or Th0 cells.It is also reported to suppress antigen-specific T cell expansion and EAE (experimental allergic encephalitis) mediated by Th1 cells.

Other Names: DNAM-1, PTA1(platelet and T cell activation antigen 1), TLISA1, LFA-1 associated Molecule PTA-1
Structure: 65 kd glycoprotein of a member of Ig-superfamily containing 2 immunologlobulin-like domains.
Distribution: Expressed on majority of CD4+ and CD8+ T cells, NK cells, monocytes/macrophages and a subset of B cells and thymocytes. Reported to be specifically expressed on the surface of Th1 cells, but downregulated in Th2 cells.
Function: Mediates cellular adhesion, involved in LFA-1 costimulatory signal for T cell differentiation and proliferation
Ligand Receptor: CD112 and CD155
Antigen References:

1.Tahara Hanaoka S, et al. 2006. Blood 107:1491
2. Shibuya K, et al. 2003. J. Exp. Med. 198:1829


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