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Safety Data Sheet (SDS) PE Anti-mouse TCR Vbeta11 Antibody     Product Data Sheet (PDF)    
PE Anti-mouse TCR Vβ11 Antibody
1229535 100 µg $190.00       
Clone: KT11
Isotype: Rat IgG2b, κ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Quigley MF, et al. 2010. Proc Natl Acad Sci USA. 9:19414. PubMed

C57BL/6 splenocytes were stained with

C57BL/6 splenocytes were stained with CD3 APC and TCR Vβ11 (clone KT11) PE (top) or rat IgG2b, κ PE isotype control (bottom).





Description:

The Vβ11 gene locus is deleted in mice of TCR a haplotype (C57BR, C57L, SJL, SWR) and TCR c haplotype (RIII).Mice of TCR b haplotype (C57BL, BALB/c, AKR, etc ) have Vβ11-bearing T cells .However, mice expressing MHC class II I-E and some superantigens, such as Mtv-8, Mtv-9 and Mtv-11 will show a partial deletion of Tb11-bearing T cells.

Other Names: T cell receptor Vβ 11 [vb11]
Distribution: Positive on a subset of alpha/beta T cells
Ligand Receptor: MHC/peptide