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Safety Data Sheet (SDS) APC/Cy7 Anti-rat CD90/mouse CD901 Thy-11 Antibody     Product Data Sheet (PDF)    
APC/Cy7 Anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody
1612595 25 µg $100.00       
1612600 100 µg $280.00       
Clone: OX-7
Isotype: Mouse IgG1, κ
Reactivity: Rat, Mouse (AKR/J, PL, and FVBIN mouse strains), Cross-Reactivity: Rabbit (Lapine), Guinea Pig
Immunogen: Rat thymocyte Thy-1 antigen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

The OX-7 antibody reacts with rat CD90 and mouse CD90.1 (Thy-1.1) (which is expressed by mouse strains of AKR/J, PL, and FVB/N), but not mouse CD90.2.

Additional reported applications (for the relevant formats) include: immunohistochemical7 and immunofluorescent8 staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, immunoprecipitation1, Western blotting1, in vitro activation of leukocytes2, induction of endothelial cell permeability3, induction of apoptosis in glomerular mesangial cells, and induction of glomerulonephritis in vivo4.

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Jeng CJ, et al. 1998. J. Cell Biol. 140:685. (IP, WB)
2. Nakashima I, et al. 1991. J. Immunol. 147:1153.
3. Ishizu A, et al. 1995. Int. Immunol. 7:1939.
4. Eitner F. 1997. Kidney. Int. 51:69.
5. Kawachi H, et al. 1992. Clin. Exp. Immunol. 88:399. (WB)
6. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
7. Daniel C, et al. 2012. Lab Invest. 92:812. (IHC)
8. Li B, et al. 2006. Kidney Int. 69:323. (IF)

LOU rat splenocytes stained with

LOU rat splenocytes stained with OX-7 APC/Cy7

LOU rat thymocytes stained with

LOU rat thymocytes stained with OX-7 APC/Cy7



Description:

CD90, also known as Thy-1, is a 28-30 kD GPI-linked membrane glycoprotein. It is a member of the immunoglobulin superfamily and has been shown to interact with CD45 in signal transduction during lymphocyte proliferation and differentiation. CD90 is expressed on hematopoietic stem cells, neurons, thymocytes, peripheral T cells, fibroblasts, stromal cells.

Other Names: Thy-1, Thy-1.1
Structure: Ig superfamily, GPI-anchored membrane glycoprotein, 28-30 kD
Distribution: Hematopoietic stem cells, thymocytes, a subset of peripheral blood T cells, early myeloid and erythroid progenitors, immature B cells, neurons, activated endothelium, mast cells, dendritic cells, glomeruli
Function: Adhesion, signal transduction, lymphocyte co-stimulation, proliferation and differentiation of hematopoietic stem cells
Ligand Receptor: Interacts with CD45
Antigen References:

1. Campbell DG, et al. 1981. Biochem. J. 195:15.
2. Hosseinzadeh H, et al. 1993. J. Immunol. 150:1670.


This product or portions thereof is manufactured under license from GE Healthcare under U.S. Patent Numbers 5,268,486; 5,569,587; 5,627,027 and patents or pending applications that are continuations, continuations-in-part, re-examinations, divisionals, reissues or foreign equivalents thereof. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.
COMMERCIAL USE shall include:

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