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Safety Data Sheet (SDS) FITC Annexin V     Product Data Sheet (PDF)    
FITC Annexin V
3804725 300 tests $0.00       
3804525 25 tests $75.00       
3804530 100 tests $160.00       
Reactivity: All mammalian species
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The purified protein was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling: The Annexin V solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Application Notes:

Annexin V Staining
1. Wash cells twice with cold cell staining buffer and then resuspend cells in Annexin V Binding Buffer at a concentration of 1x10e6 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of PI solution or 7-AAD.
5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry.

Recommended Usage:

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 100,000 - million cells in a 100 µl volume of Annexin V Binding Buffer (Cat No. 422201). It is recommended that the reagent be titrated for optimal performance for each application.

Application References:

1. Koopman G, et al. 1994. Blood 84:1415.
2. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
3. Dachary-Prigent J, et al. 1993. Blood 81:2554.
4. Sekine C, et al. 2009. Int Immunol. PubMed
5. Grujic M, et al. 2010. J. Immunol. 185:1730. PubMed
6. Mulik RS, et al. 2010. Int J Pharm. 398:190. PubMed
7. Gupta A, et al. 2011. Leuk Res. 35:1498. PubMed
8. Speth C,et al. 2013. J Infect Dis. 207:823.  PubMed
9. Wang Q, et al. 2013. Genes Dev. 27:615. PubMed
10. Gill K, et al. 2013. Biocheim Biophys Acta. 830:2763. PubMed
11. Juel HB, et al. 2013. PLoS One. 8:64619. PubMed
12. Hellmann J, et al. 2013. J. Immunol. 191:1383. PubMed
13. Sequier S, et al. 2013. PLoS One. 8:70937. PubMed
14. Galski H, et al. 2013. Biochem Pharmacol. 86:584. PubMed
15. Chen YL, et al. 2013. J Exp Med. PubMed
16. Lin WH, et al. 2014. PLoS One. 9:83160. PubMed
17. Salge-Bartles U, 2014. Thromb res. 133:244. PubMed
18. Zhang X, et al. 2014. Acta Biochim Biophys Sin. 46:261. PubMed
19. Intermann J, et al. 2014. Cell Signal. 26:1567. PubMed
20. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
21. M Parrula MC, et al. 2014. Virus Res. 189:206. PubMed
22. Passaro C, et al. 2014. Mol Oncol. 9:78. PubMed
23. Tsou WI, et al. 2014. J Biol Chem. 289:25750. PubMed
24. Awojoodu AO, et al. 2014. Blood. 124:1941. PubMed
25. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
26. Liu J, et al. 2015. J Mol Cell Cardiol. 80:10. PubMed

Human T leukemia cell line,

Human T leukemia cell line, Jurkat, non-treated (top) or treated (bottom) with anti-human CD95 (EOS9.1) mAb for 6 hours, then stained with Annexin V-FITC and Propidium Iodide (PI)





Description:

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining.Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. So, we recommend using our 7-AAD Viability Staining Solution or Propidium Iodide Solution . Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

Other Names: Annexin A5